Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 13 5 2021 10 08 664 670 Mahdiyar Iravani Saadi Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Abbas Doosti Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran Heeva Jalali Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran Ehsan Nabi Abdolyousefi Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Mansooreh Hooshiyar Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran Reza Tabrizi Non-Communicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran Esmat Noshadi Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran 2021 08 16 2021 08 22 Background and Objectives: Phytase has a hydrolysis function of phytic acid, which yields inorganic phosphate. Bacillus species can produce thermostable alkaline phytase. The aim of this study was to isolate and clone a Phytase gene (Phy) from Bacillus subtilis in Escherichia coli. Materials and Methods: In this study, the extracellular PhyC gene was isolated from Bacillus subtilis Phytase C. After purification of the bands, DNA fragment of Phy gene was cloned by T/A cloning technique, and the clone was transformed into Escherichia coli. Afterward, the pGEM-Phy was transferred into E. coli Top-10 strain and the recombinants were plated on LB agar containing 100 µg/ml ampicillin. The colonization of 1171 bp of gene Phytase C was confirmed by PCR. The presence of gene-targeting in vector was confirmed with enzymatic digestion by XhoI and XbaI restriction enzymes. Results: The Phytase gene was successfully cloned in E. coli. The result of cloning of 1171 bp Phytase gene was confirmed by PCR assay. Conclusion: Our impression of this article is that several methods, such as using along with microbial, plant phytase reproduction, or low-phytic acid corn may be the better way from a single phytase. https://ijm.tums.ac.ir/index.php/ijm/article/view/3283 https://ijm.tums.ac.ir/index.php/ijm/article/download/3283/1391