Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 13 5 2021 10 08 565 573 Özgür Appak Department of Medical Microbiology, Division of Medical Virology, School of Medicine, Dokuz Eylul University, Izmir, Turkey Abdurrahman Gülmez Department of Medical Microbiology, Istanbul Basaksehir Cam ve Sakura Hospital, Istanbul, Turkey Irmak Güzel Department of Medical Microbiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey Naciye Gezer Department of Radiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey Özlem Gürsoy Doruk Department of Medical Biochemistry, School of Medicine, Dokuz Eylul University, Izmir, Turkey Osman Ergor Department of Public Health, School of Medicine, Dokuz Eylul University, Izmir, Turkey Nuran Esen Department of Medical Microbiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey Ayca Sayıner Department of Medical Microbiology, Division of Medical Virology, School of Medicine, Dokuz Eylul University, Izmir, Turkey 2021 04 24 2021 08 06 Background and Objectives: In this study, the performance of three different commercial antibody assays for COVID-19 was examined and parameters affecting the antibody response were investigated. The correlation of patients’ chest CT results, procalcitonin, CRP, and D-dimer levels with the antibody response were retrospectively evaluated. Materials and Methods: COVID-19 antibodies were detected by three commercially available assays in each patient. Two of the assays were rapid immunochromatographic tests and - one was an ELISA-based IgG assay. SARS-CoV-2 RNA was tested by “COVID-19 RT-qPCR Detection Kit” using nasopharyngeal swab samples. The results of antibody tests were compared with each other, RT-qPCR, Biochemical parameters and chest CT findings. Results: RT-qPCR was positive in 46.6% (41/88) of the evaluated patients among which 77.3% (68/88) were healthcare workers. Seventeen (41.4%) of viral RNA positive patients had a positive antibody result with at least two assays. Both of the rapid immunochromatographic tests had identical sensitivity of 36.6% and specificity of 100%, compared to RT-qPCR assay; while the sensitivity of the ELISA based Euroimmune test was 43.9%, and the specificity was 95.7%. The sensitivity and specificity of the immunochromatographic tests were 75% and 100% respectively, compared to ELISA test result. There was a correlation between antibody positivity and old age and male gender. The presence of typical chest CT findings increased the antibody positivity 13.62 times. Antibody positivity was also increased with the decrease in Ct value of the PCR assay. There was no significant relationship between the biochemical parameters (CRP, D-dimer and procalcitonin values) and the antibody or RT-qPCR results. Conclusion: There was a correlation between antibody response and male gender, older age, presence of symptoms, typical chest CT findings and low PCR-Ct value. https://ijm.tums.ac.ir/index.php/ijm/article/view/3114 https://ijm.tums.ac.ir/index.php/ijm/article/download/3114/1380