Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 13 4 2021 08 11 458 463 Rasoul Ghaioumy Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranPharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; Clinical Research Development Unit, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran Fatemehalsadat Tabatabaeifar Department of Chemical Engineering, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, UANL, San Nicolás de los Garza, Mexico; Centro de Investigaciónen Biotecnología y Nanotecnología, Facultad de CienciasQuímicas, Parque de Investigación e Innovación Tecnológica, Universidad Autónoma de Nuevo León, Apodaca, Mexico Karamat Mozafarinia Clinical Research Development Unit, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran Aliasghar Arabi Mianroodi Clinical Research Development Unit, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran Elham Isaei Student Research Committee, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran José Rubén Morones-Ramírez Department of Chemical Engineering, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, UANL, San Nicolás de los Garza, Mexico; Centro de Investigaciónen Biotecnología y Nanotecnología, Facultad de CienciasQuímicas, Parque de Investigación e Innovación Tecnológica, Universidad Autónoma de Nuevo León, Apodaca, Mexico Setareh Agha Kuchak Afshari Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran; Department of Medical Parasitology and Mycology, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran Davood Kalantar-Neyestanaki Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran; Department of Medical Microbiology (Bacteriology & Virology), Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran 2021 04 04 2021 06 05 Background and Objectives: It is well known that Staphylococcus aureus biofilm plays an important role in adenoiditis and biofilm resistance frequently results in failure of therapy. The goal of this study was to evaluate the biofilm production of S. aureus isolates obtained from adenoid specimens and assess the relationship between biofilm formation ability and ica operon genes. Materials and Methods: A total of 112 adenoid samples were obtained from patients under 15 years old with adenoid hypertrophy. All S. aureus isolates were initially identified by standard microbiological tests and amplification of nuc by polymerase chain reaction (PCR) technique. Biofilm formation of S. aureus isolates was evaluated and icaADBC genes were detected by PCR technique. Results: There were 46 isolates (41%) identified as S. aureus. The ability to produce biofilm was detected among total S. aureus isolates. Molecular study of ica operon revealed that 2 (6.3%) and 19 (59.4%) isolates carried icaA and icaD, respectively. The prevalence of icaA + icaD was seen among 11 (34.4%) S. aureus isolates, while icaC and icaB were not detected. Conclusion: Our findings indicated that icaABCD operon are associated with biofilm formation in S. aureus isolates, however the absence of these genes may not necessarily exclude this property. https://ijm.tums.ac.ir/index.php/ijm/article/view/3073 https://ijm.tums.ac.ir/index.php/ijm/article/download/3073/1366