Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 14 1 2022 02 20 112 118 EN Alireza Tabibzadeh Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Mahshid Panahi Gastrointestinal and Liver Diseases Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Behnaz Bouzari Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Mohammad Taghi Haghi Ashtiani Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Farhad Zamani Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, IranGastrointestinal and Liver Diseases Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Hadi Teimoori Arzati Department of Veterinary, Islamic Azad University, Karaj Branch, Karaj, Iran Mohammad Hadi Karbalaie Niya Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Gastrointestinal and Liver Diseases Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran 2021 02 18 2021 10 05 Background and Objectives: The human papillomavirus (HPV) is associated with more than 70% of the cervical neoplasm. The current study aims to evaluate the distribution of HPV genotypes in suspected women cytological specimens from Tehran, Iran. Materials and Methods: In the current cross-sectional study, HPV genotype prevalence was investigated in 433 subject women. DNA extraction was performed by High Pure Viral Nucleic Acid kit. A semi-automatically hybriSpot 24™ (HS24) setting was used for HPV typing and data interpreted by hybriSoft™ software according to instructions. Results: Pathologic data showed 181 (41.8%) had non-malignant lesions, 212 (49%) had inflammation and 40 (9.2%) reported LSIL in primary Pap-smear result. HPV was found in 143 (33%) specimens and the most comment high-risk and low-risk HPV types were HPV-16 and -6, respectively. Also, 62 (43%) were co-infected with multiple genotypes includes, 34 (24%) cases had co-infection with two HPV types, 17 (12%) cases had co-infection with three HPV types, 6 (4%) cases had co-infection with four HPV types and 5 (3%) cases had co-infection with five HPV types. There was statistically different domination on high-risk genotype in most of the co-infected samples (p<0.01). Conclusion: Current study indicates that the lesion pathology assessment was significantly associated with the HPV infection (p<0.01). Furthermore, the age group assessment shows that most of the HPV positive cases were 21 to 40 (p<0.01). The HPV infection prevalence in the current study was 33% and the most frequently reported high-risk and low-risk HPV types were 16 and 6, respectively. https://ijm.tums.ac.ir/index.php/ijm/article/view/2984 https://ijm.tums.ac.ir/index.php/ijm/article/download/2984/1432