Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 11 6 2020 01 11 468 477 Ibrahim Taher Department of Pathology, College of Medicine, Jouf University, Sakaka, Saudi Arabia Abdulrahman Almaeen Department of Pathology, College of Medicine, Jouf University, Sakaka, Saudi Arabia Hassan Aljourfi Department of Medicine,College of Medicine, Jouf University, Sakaka, Saudi Arabia Eyad Bohassan Department of Medicine,College of Medicine, Jouf University, Sakaka, Saudi Arabia Ahmed Helmy Department of Tropical Medicine & Gastroenterology, Assiut University Hospitals, Assiut, Egypt Eman El-Masry Department of Pathology, College of Medicine, Jouf University, Sakaka, Saudi Arabia Baraka Saleh Department of Microbiology, Microbiology Laboratory, Prince Mutaib Hospital, Sakaka, Saudi Arabia Nawaf Aljaber Department of Microbiology, Microbiology Laboratory, Prince Mutaib Hospital, Sakaka, Saudi Arabia 2019 10 06 2019 11 30 Background and Objectives: Urinary tract infections are common health problem affecting millions worldwide. Antibiotic resistance among uropathogens (Ups) is prevalent in many countries. In the absence of any available data in the region, this hospital-based study investigated the pattern, frequency and susceptibility of Ups at Prince Mutaib Bin Abdulaziz Hospital, Aljouf Region, Saudi Arabia. Materials and Methods: A retrospective assessment of UPs and their antibiotics susceptibility was conducted from January 2017 to December 2017 using the fully automated Vitek2 system (BioMérieux, France). Results: Among the 415 uropathogens isolates, the most prevalent bacteria were Gram-negatives comprising 137 (51%) E. coli; 46 (17.2%) Klebsiella spp.; 30 (11.2%) Pseudomonas spp.; 25 (9.3%) Proteus spp.; 14 (5.2%) Acinetobacter baumanii and 16 (5.9%) others. On the other hand, Enterococcus spp. were predominant among Gram-positive isolates representing 54 (36.7%), 47 (32.0%) Staphylococcus spp., 22 (15.1%) Streptococcus spp., and 13 (8.8%) S. aureus, and 11 (7.5%) others. Gram-negative Ups showed multidrug resistance towards the majority of the tested antimicrobials (ampicillins, cephalosporins, fluoroquinolones, trimethoprim-sulfamethoxazole, fosfomycin, aztreonam, and nitrofurantoin). While high resistance patterns by Gram-positives was also seen against cephalosporins, penicillins, amoxicillin-clavulanic acid, trimethoprim-sulfamethoxazole, clindamycin, erythromycin and tetracycline. Conclusion: The observed widespread multidrug resistance clearly warrant implementing stricter control measures, local guidelines of antimicrobials usage, and continuous epidemiological surveys at hospitals and communities. https://ijm.tums.ac.ir/index.php/ijm/article/view/2351 https://ijm.tums.ac.ir/index.php/ijm/article/download/2351/1203