Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 11 5 2019 11 12 389 396 Pratiwi Kusumo Biomedic Doctoral Programme, School of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Biomedic, School of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia Hasan Maulahela Department of Gastroenterology, School of Medicine, Universitas Indonesia, Jakarta, Indonesia Amanda Utari Department of Gastroenterology, School of Medicine, Universitas Indonesia, Jakarta, Indonesia Ingrid Surono Department of Food Technology, Faculty of Engineering, Bina Nusantara University, Jakarta, Indonesia Amin Soebandrio Eijkman Biology Molecular Institute, Jakarta, Indonesia; Department of Microbiology, School of Medicine, Universitas Indonesia, Jakarta, Indonesia Murdani Abdullah Department of Gastroenterology, School of Medicine, Universitas Indonesia, Jakarta, Indonesia 2019 06 19 2019 08 30 Background and Objectives: Gut microbiota influences our health via multiple mechanisms. Microbiota produced Short Chain Fatty Acid (SCFA) as an energy to maintain gut ecosystem and physiology. Dysbiosis is correlated with SCFA imbalance which in turn resulted in physiological abnormalities in the intestine, such as functional constipation. Materials and Methods: Randomized Double-Blind Controlled Trial (RCT) was conducted on women with functional constipation (n=37) in the community of Jakarta and profile of SCFA was assessed by using GC-MS from the stool after 21 days supplementation of fermented milk (placebo and probiotic). Results: Probiotic supplementation significantly influenced acetate titer (p=0,032) marginally significant for propionate and butyrate (p=0.063 and p=0.068, respectively) and the respondent with increasing SCFA’s metabolite are higher in probiotic group compared to the respondents in placebo group. Acetate is the highest SCFA titer found in faeces samples of women with functional constipation. Conclusion: Probiotic Lactobacillus plantarum IS 10506 supplementation influenced all the SCFA parameter (acetate, propionate and butyrate). https://ijm.tums.ac.ir/index.php/ijm/article/view/2218 https://ijm.tums.ac.ir/index.php/ijm/article/download/2218/1194