Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 10 5 2018 11 23 300 306 EN Mohadeseh Zarei-Yazdeli Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Gilda Eslami Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Hengameh Zandi Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Masoumeh Kiani Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Kazem Barzegar English Language Department at Medical School of Shahid Sadoughi University of Medical Sciences, Yazd, Iran Hanieh Alipanah Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran Seyed Morteza Mousavi Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Marzieh Shukohifar Department of Biostatistics and Epidemiology, Faculty of Paramedicine Abarkouh, Genetic and Environmental Adventures Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 2018 05 18 2018 08 16 Background and Objectives: Antibiotic resistance in Pseudomonas aeruginosa is an increasing health problem. Integrons are associated with a variety of gene cassettes, which confer resistance to multiple classes of antibiotics. This study aimed at screening the presence of class 1, 2 and 3 integrons in P. aeruginosa in Yazd, Iran. Materials and Methods: This study was carried out on P. aeruginosa strains from March 2016 to March 2017. Clinical specimens were initially identified by the standard biochemical methods and their resistance patterns to antibiotics were studied using the disc diffusion method. PCR was carried out for the detection of class 1, 2 and 3 integrons using intI1, intI2 and intI3 gene primers, respectively. Results: Antimicrobial susceptibility test showed that 75% of isolates were detected as multi-drug resistant (MDR), and lowest resistance was observed in ciprofloxacin (48.6%) and most resistance was in gentamicin (63.2%). Moreover, PCR results showed that 22 (15.3%) and 119 (82.6%) of P. aeruginosa isolates carried intI2 and intI1 genes, but intI3 gene was not found. Conclusion: Since it is customary to observe Class I integrons in P. aeruginosa isolated from clinical samples, they are often responsible for antibiotic resistance gene transfer, which calls for evaluation of integrons as contributing factors in antibiotic resistance. https://ijm.tums.ac.ir/index.php/ijm/article/view/1752 https://ijm.tums.ac.ir/index.php/ijm/article/download/1752/1070