Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 8 2 2016 05 14 132 138 EN Roghayeh Teimourpour Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. Hosna Zare Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Ramazan Rajabnia Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran. Yousef Yahyapoor Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran. Zahra Meshkat Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. 2015 10 03 2016 03 29 Background and Objectives: HBHA and Mtb32C have been isolated from culture supernatants of Mycobacterium tu- berculosis (M. tuberculosis) and Mycobacterium bovis (M. bovis) and their immunogenicity previously studies have been confirmed. In this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (Mt- b32C-HBHA), in producing new chimeric protein under the in-vitro condition was examined. Materials and Methods: In present study Huh7.5 cells was transfected with Mtb32C-HBHA –pCDNA3.1+ recombinant vector using the calcium phosphate method and expression of chimeric protein was assessed by RT-PCR and Western blot methods. Results: Results of RT-PCR and Western blot showed expression of 35.5 KD recombinant protein (Mtb32C-HBHA) in thiscell line. Conclusion: The constructed vector can produce two highly immunogenic antigens that fusion of them to gather makes chi- meric antigen with new traits. Other attempts are needed to evaluate specific properties of this new antigen such as molecular conformation modeling and immunologic characteristics in future studies. https://ijm.tums.ac.ir/index.php/ijm/article/view/118 https://ijm.tums.ac.ir/index.php/ijm/article/download/118/583