Iranian Journal of Microbiology 2017. 9(3):160-168.

Evaluation of anti-bacterial effects of nickel nanoparticles on biofilm production by Staphylococcus epidermidis
Morteza Vahedi, Nima Hosseini-Jazani, Saber Yousefi, Maryam Ghahremani

Abstract


Background and Objectives: Staphylococcus epidermidis produces biofilm by extracellular polysaccharides, causing bacterial adherence to different surfaces. Anti-microbial effects of nickel nanoparticles on some bacterial strains such as S. aureus and Escherichia coli have been determined in limited studies. The aim of the present study is to examine the inhibitory effect of nickel nanoparticles on biofilm formation using clinical isolates of S. epidermidis and its hemolytic effect on human red blood cells.
Materials and Methods: Twenty two S. epidermidis isolates were collected and identified by standard microbiological methods. Microtiter plate method was used to determine the biofilm production in bacterial isolates . The amounts of biofilm formation by isolates in the presence of 0.01, 0.05, 0.1, and 1 mg/mL concentrations of nickel nanoparticles were measured. Hemolytic activity of different concentrations of nickel nanoparticles was measured on human RBC suspensions.
Results: Twenty isolates were strong, and two isolates were moderate biofilm producers. Biofilm formation significantly decreased in the presence of 0.05, 0.1, and 1 mg/mL of nickel nanoparticles (p<0.05). Although in the presence of 0.01 mg/mL of nickel nanoparticles, decrease in biofilm formation was observed but it was not statistically significant (p=0.448). Slight hemolytic activity was seen in the presence of nickel nanoparticles.
Conclusion: In this study, the ability of biofilm production was demonstrated for all clinical isolates of S. epidermidis. On the other hand, the lowering effects of nickel nanoparticles on biofilm formation were observed.


Keywords


Staphylococcus epidermidis, Nickel nanoparticles, Biofilm

Full Text:

PDF

References


Fey PD, Olson ME. Current concepts in biofilm formation of Staphylococcus epidermidis. Future Microbiol 2010; 5: 917-933.

Namvar AE, Bastarahang S, Abbasi N, Ghehi GS, Farhadbakhtiarian S, Arezi P, et al. Clinical characteristics of Staphylococcus epidermidis: a systematic review. GMS Hyg Infect Control 2014; 9: Doc23.

Buttner H, Mack D, Rohde H. Structural basis of Staphylococcus epidermidis biofilm formation: mechanisms and molecular interactions. Front Cell Infect Microbiol 2015;5:14.

Ceri H, Olson ME, Stremick C, Read RR, Morck D, Buret A. The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. ‎J Clin Microbiol 1999; 37:1771-1776.

Otto M. Staphylococcus epidermidis-the 'accidental' pathogen. ‎Nature Rev Microbiol 2009; 7: 555-567.

McLean RJ, Lam JS, Graham LL. Training the Biofilm Generation--a tribute to J. W. Costerton. ‎J Bacteriol 2012; 194: 6706-6711.

Williams DL, Costerton JW. Using biofilms as initial inocula in animal models of biofilm-related infections. J Biomed Mater Res B Appl Biomater 2012; 100:1163-1169.

Chen X, Schluesener H. Nanosilver: a nanoproduct in medical application. Toxicol Lett 2008; 176:1-12.

Horikoshi S, Serpone N (2013). Introduction to nanoparticles. Microwaves in Nanoparticle Synthesis: Fundamentals and Applications. 1 nd ed. Wiley online library.

Wilde G ( 2009). Nanostructured materials. 1nd ed. Elsevier. Boston.

Rudramurthy GR, Swamy MK, Sinniah UR, Ghasemzadeh A. Nanoparticles: alternatives against drug-resistant pathogenic microbes. Molecules 2016; 27;21(7).

Coates A, Hu YM, Bax R, Page C. The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discov 2002; 1: 895-910.

Mu H, Tang J, Liu Q, Sun C, Wang T, Duan J. Potent antibacterial nanoparticles against biofilm and intracellular Bacteria. Sci Rep 2016; 6:18877.

Shi SF, Jia JF, Guo XK, Zhao YP, Chen DS, Guo YY, et al. Reduced Staphylococcus aureus biofilm formation in the presence of chitosan-coated iron oxide nanoparticles. Int J Nanomedicine 2016;11:6499-6506.

Singh A, Ahmed A, Prasad KN, Khanduja S, Singh SK, Srivastava JK, et al. Antibiofilm and membrane-damaging potential of cuprous oxide nanoparticles against Staphylococcus aureus with reduced susceptibility to vancomycin. Antimicrob Agents Chemother 2015; 59: 6882-6890.

Ali K, Ahmed B, Dwivedi S, Saquib Q, Al-Khedhairy AA, Musarrat J. Microwave accelerated green synthesis of stable silver nanoparticles with eucalyptus globulus leaf extract and their antibacterial and antibiofilm activity on clinical isolates. PloS one 2015; 10: e0131178.

Lambadi PR, Sharma TK, Kumar P, Vasnani P, Thalluri SM, Bisht N, et al. Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control. Int J Nanomedicine 2015;10:2155-2171.

Argueta-Figueroa L, Morales-Luckie RA, Scougall-Vilchis RJ, Olea-Mejia OF. Synthesis, characterization and antibacterial activity of copper, nickel and bimetallic Cu-Ni nanoparticles for potential use in dental materials. Prog Nat Sci-Mater 2014; 24: 321-328.

Webster TJ (2009). Safety of nanoparticles : from manufacturing to medical applications. 1nd ed. Springer. New York.

Forbes BA, Sahm DF, Weissfeld AS (2007). Bailey & Scott's Diagnostic Microbiology. 12nd ed. Mosby Elsevier. St. Louis

O'Toole GA. Microtiter dish biofilm formation assay. J Vis Exp 2011; 30: 2437.

Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 2007;115: 891-899.

Kalishwaralal K, BarathManiKanth S, Pandian SR, Deepak V, Gurunathan S. Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids Surf B Biointerfaces 2010; 79:340-344.

Jiang L, Yu Y, Li Y, Yu Y, Duan J, Zou Y, et al. Oxidative damage and energy metabolism disorder contribute to the hemolytic effect of amorphous silica nanoparticles. Nanoscale Res Lett 2016; 11:57.

Kamerud KL, Hobbie KA, Anderson KA. Stainless steel leaches nickel and chromium into foods during cooking. ‎J Agric Food Chem 2013; 61: 9495-9501.

Hoffmann W, Bormann T, Rossi A, Muller B, Schumacher R, Martin I, et al. Rapid prototyped porous nickel-titanium scaffolds as bone substitutes. J Tissue Eng 2014;5:2041731414540674.

Pulikkottil VJ, Chidambaram S, Bejoy PU, Femin PK, Paul P, Rishad M. Corrosion resistance of stainless steel, nickel-titanium, titanium molybdenum alloy, and ion-implanted titanium molybdenum alloy archwires in acidic fluoride-containing artificial saliva: An in vitro study. J Pharm Bioall Sci 2016; 8(Suppl 1):S96-S9.

Dhanyalayam D, Scrivano L, Parisi OI, Sinicropi MS, Fazio A, Saturnino C, et al. Biopolymeric self-assembled nanoparticles for enhanced antibacterial activity of Ag-based compounds. Int J Pharm 2016; 517: 395-402.

Patil MP, Kim GD. Eco-friendly approach for nanoparticles synthesis and mechanism behind antibacterial activity of silver and anticancer activity of gold nanoparticles. Appl Microbiol Biotechnol 2017; 10: 79-92.

Gopinath K, Kumaraguru S, Bhakyaraj K, Mohan S, Venkatesh KS, Esakkirajan M, et al. Green synthesis of silver, gold and silver/gold bimetallic nanoparticles using the Gloriosa superba leaf extract and their antibacterial and antibiofilm activities. Microb Pathog 2016; 101:1-11.

Essa AM, Khallaf MK. Antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles. BMC Microbiol 2016; 16: 144.

Choi HJ, Choi JS, Park BJ, Eom JH, Heo SY, Jung MW, et al. Enhanced transparency, mechanical durability, and antibacterial activity of zinc nanoparticles on glass substrate. Sci Rep 2014; 4: 6271.

Aldujaili NH, Abdullah NY, Khaqani RL, Al-tfaly SA, Al-Shammary AH. Biosynthesis and antibacterial activity of titanium nanoparticles using Lactobacillus. Int J Recent Sci Res 2015; 6: 7741-7751.

Mamonova I. Study of the antibacterial action of metal nanoparticles on clinical strains of Gram negative bacteria. World J Med Sci 2013; 8: 314.

Ashtari K, Fasihi J, Mollania N, Khajeh K. A biotemplated nickel nanostructure: Synthesis, characterization and antibacterial activity. Mater Res Bull 2014; 50: 348-353.

Lara HH, Romero-Urbina DG, Pierce C, Lopez-Ribot JL, Arellano-Jimenez MJ, Jose-Yacaman M. Effect of silver nanoparticles on Candida albicans biofilms: an ultrastructural study. J Nanobiotechnology 2015;15: 91.

Loo CY, Rohanizadeh R, Young PM, Traini D, Cavaliere R, Whitchurch CB, et al. Combination of silver nanoparticles and curcumin nanoparticles for enhanced anti-biofilm activities. J Agric Food Chem 2016; 64: 2513-2522.

Fabrega J, Zhang R, Renshaw JC, Liu WT, Lead JR. Impact of silver nanoparticles on natural marine biofilm bacteria. Chemosphere 2011; 85: 961-966.

Ziebuhr W, Hennig S, Eckart M, Kranzler H, Batzilla C, Kozitskaya S. Nosocomial infections by Staphylococcus epidermidis: how a commensal bacterium turns into a pathogen. Int J Antimicrob Agents 2006; 28 Suppl 1:S14-20.

Solati SM, Tajbakhsh E, Khamesipour F, Gugnani HC. Prevalence of virulence genes of biofilm producing strains of Staphylococcus epidermidis isolated from clinical samples in Iran. AMB Express 2015;5:134.

Perrin C, Briandet R, Jubelin G, Lejeune P, Mandrand-Berthelot MA, Rodrigue A, et al. Nickel promotes biofilm formation by Escherichia coli K-12 strains that produce curli. Appl Environ Microbiol 2009; 75: 1723-1733.

Buzea C, Pacheco, II, Robbie K. Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2007; 2: MR17-71.

Besinis A, De Peralta T, Handy RD. The antibacterial effects of silver, titanium dioxide and silica dioxide nanoparticles compared to the dental disinfectant chlorhexidine on Streptococcus mutans using a suite of bioassays. Nanotoxicology 2014; 8: 1-16.

Brayner R, Ferrari-Iliou R, Brivois N, Djediat S, Benedetti MF, Fievet F. Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. Nano letters 2006; 6: 866-8670.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.