Iranian Journal of Microbiology 2016. 8(5):298-306.

Multi-locus sequence type analysis of Shigellas pp. isolates from Tehran, Iran
shadi shahsavan, Maliheh Nobakht, Abdolaziz Rastegar-Lari, Parviz Owlia, Bita Bakhshi


Background and Objectives: Strains of Shigella spp. can cause shigellosis, or bacillary dysentery.that is a public health problem worldwide. The aim of this study was to describe the population structure and genetic relatedness of multidrug resistant S. sonnei and S. flexneri isolated during a one year period from children with diarrhea in Tehran, Iran.

Materials and Methods: A total of 70 Shigella spp. were detected during the study period . Twenty MDR isolates of Shigella spp. were randomly selected and used in this study. Bacterial identification was performed by conventional biochemical and serological and confirmed by molecular method. After antimicrobial susceptibility testing, we used Multilocus sequence typing (MLST) for subtyping isolates.

Results: We found 14 Shigella sonnei and 6 Shigella flexneri isolates. Results of MLST showed five sequence types (ST) (145, 152, 241, 245, 1502) and BURST analysis revealed the largest number of single locus variant (SLV) and highest frequency (FREQ) for ST152. ST 152 with nine members was predicted as the founder by BURST. Frequency for ST 1502 and ST 245 was four isolates and the least frequency was seen for ST 241 and 145 with one and two members, respectively. ST 145 and ST 245 were described as singletons in BURST. All isolates with ST145 and ST245 were identified as Shigella flexneri.

Conclusion: Annual Multi locus sequence typing of MDR Shigella would help us in better understanding of dominant species and comparing our results with the same studies in other countries especially our neighbor countries in source tracking purposes.

Keywords: Shigella, Multilocus sequence typing, Multidrug resistant


Shigella; Multilocus sequence typing; Multidrug resistant

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Todar K. Todar-s online textbook of bacteriology. Uni- versity of Wisconsin-Madison Department of Bacteri- ology. 2005.

Heiman KE, Karlsson M, Grass J, Howie B, Kirkcaldy RD, Mahon B, et al. Notes from the field: Shigella with decreased susceptibility to azithromycin among men who have sex with men-United States, 2002-2013. MMWR Morb Mortal Wkly Rep 2014;63:132–133.

Seidlein L von, Kim DR, Ali M, Lee H, Wang X, Thiem VD, et al. A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifesta- tions, and microbiology. PLoS Med 2006;3:e353–e353.

Priest F, Ramos-Cormenzana A, Tindall BJ. Bacterial diversity and systematics. Springer Science & Business Media; 2012.

Pazhani GP, Ramamurthy T, Mitra U, Bhattacharya SK, Niyogi SK. Species diversity and antimicrobial resistance of Shigella spp. isolated between 2001 and 2004 from hospitalized children with diarrhoea in Kol- kata (Calcutta), India. Epidemiol Infect 2005;133:1089–1095.

Rowe-Magnus DA, Guerout A-M, Mazel D. Bacterial resistance evolution by recruitment of super-integron gene cassettes. Mol Microbiol 2002;43:1657–1669.

Sire J-M, Macondo EA, Perrier-Gros-Claude J-D, Siby T, Bahsoun I, Seck A, et al. Antimicrobial resistance in Shigella species isolated in Dakar, Senegal (2004-2006). Jpn J Infect Dis 2008;61:307–309.

Choi SY, Jeon Y-S, Lee JH, Choi B, Moon SH, von Se- idlein L, et al. Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries. J Med Microbiol 2007;56:1460–6.

Holt KE, Baker S, Weill F-X, Holmes EC, Kitchen A, Yu J, et al. Shigella sonnei genome sequencing and phylogenetic analysis indicate recent global dissemina- tion from Europe. Nat Genet 2012;44:1056–1059.

Spratt BG. Multilocus sequence typing: molecular typing of bacterial pathogens in an era of rapid DNA sequencing and the internet. Curr Opin Microbiol 1999;2:312–316.

Inouye M, Conway TC, Zobel J, Holt KE. Short read sequence typing (SRST): multi-locus sequence types from short reads. BMC Genomics 2012;13:338.

Services M. UK Standards for Microbiology Investiga- tions. Bacteriology 2014;B 55:1–21.

Toma C, Lu Y, Higa N, Nakasone N, Chinen I, Baschki- er A, et al. Multiplex PCR assay for identification of hu- man diarrheagenic Escherichia coli. J Clin Microbiol 2003;41:2669–2671.

Ojha SC, Yean Yean C, Ismail A, Banga Singh K-K.A pentaplex PCR assay for the detection and dif- ferentiation of Shigella species. Biomed Res Int 2013;2013:412370.

Wirth T, Falush D, Lan R, Colles F, Mensa P, Wieler LH, et al. Sex and virulence in Escherichia coli: an evolu- tionary perspective. Mol Microbiol 2006;60:1136–1151.

Feil EJ, Li BC, Aanensen DM, Hanage WP, Spratt BG. eBURST: inferring patterns of evolutionary de- scent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol2004;186:1518–1530.

Eftekhari N, Bakhshi B, Pourshafie MR, Zarbakhsh B, Rahbar M, Hajia M, et al. Genetic diversity of Shigella spp. and their integron content. Foodborne Pathog Dis 2013;10:237–242.

Mardaneh J, Poor SA, Afrugh P. Prevalence of Shigella species and antimicrobial resistance patterns of isolat- ed strains from infected pediatrics in Tehran. Int J En- tric Pathog 2013;1:28–31.

Ranjbar R, Dallal MMS, Talebi M, Pourshafie MR.Increased isolation and characterization of Shigella sonnei obtained from hospitalized children in Tehran, Iran. J Health Popul Nutr 2008;26:426.

Eliopoulos GM, Huovinen P. Resistance to trimetho- prim-sulfamethoxazole. Clin Infect Dis 2001;32:1608–1614.

Pourakbari B, Mamishi S, Mashoori N, Mahboobi N, Ashtiani MH, Afsharpaiman S, et al. Frequency and an- timicrobial susceptibility of Shigella species isolated in Children Medical Center Hospital, Tehran, Iran, 2001-2006. Braz J Infect Dis 2010 Mar-Apr;14(2):153-157.

Khan S, Singh P, Ansari M, Asthana A. Isolation of Shigella species and their resistance patterns to a panel of fifteen antibiotics in mid and far western region of Nepal. Asian Pacific J Trop Dis 2014;4:30–34.

Toro C, Arroyo A, Sarria A, Iglesias N, Enríquez A, Baquero M, et al. Shigellosis in subjects with traveler’s diarrhea versus domestically acquired diarrhea: impli- cations for antimicrobial therapy and human immu- nodeficiency virus surveillance. Am J Trop Med Hyg 2015;93:491–496.

Iqbal MS, Rahman M, Islam R, Banik A, Amin MB, Akter F, et al. Plasmid-mediated sulfamethoxazole re- sistance encoded by the sul2 gene in the multidrug-re- sistant Shigella flexneri 2a isolated from patients with acute diarrhea in Dhaka, Bangladesh. PLoS One 2014;9:e85338.

Barman S, Chatterjee S, Chowdhury G, Ramamurthy T, Niyogi SK, Kumar R, et al. Plasmid-mediated strep- tomycin and sulfamethoxazole resistance in Shigella flexneri 3a. Int J Antimicrob Agents 2010;36:348-351.

Bowen A, Hurd J, Hoover C, Khachadourian Y, Trap- hagen E, Harvey E, et al. Importation and domestic transmission of Shigella sonnei resistant to ciproflox- acin-United States, May 2014-February 2015. MMWR Morb Mortal Wkly Rep 2015;64:318–320.

Gharibi O, Zangene S, Mohammadi N, Mirzaei K, Karimi A, Gharibi A, et al. Increasing antimicrobial resistance among Shigella isolates in the Bushehr, Iran. Pak J Biol Sci 2012 Feb 1;15(3):156-159.

Jeon Y La, Nam Y, Lim G, Cho SY, Kim Y-T, Jang J-H, et al. Quinolone-resistant Shigella flexneri isolat- ed in a patient who travelled to India. Ann Lab Me 2012;32:366–369.

Cao Y, Wei D, Kamara IL, Chen W. Multi-Locus Se- quence Typing (MLST) and Repetitive Extragenic Palindromic Polymerase Chain Reaction (REP-PCR), characterization of shigella spp. over two decades in Tianjin China. Int J Mol Epidemiol Genet 2012;3(4) :321-332.


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