Iranian Journal of Microbiology 2016. 8(6):372-376.

The antibacterial activity of methanolic Anacyclus pyrethrum and Pistacia lentiscus L. extract on Escherichia coli
Noushin Jalayer Naderi, Mohhamad Niakan, Elham khodadadi, Maryam Mohamadi Motlagh

Abstract


Background and Objectives: Antibiotic therapy is the main choice in treatment of Escherichia coli induced infections. Using herbal medication is an alternative choice in treatment of diseases. The aim of this study was to determine the antibacterial activity of two traditionally used herbs in Iranian medicine, Anacyclus pyrethrum and Pistacia lentiscus L., on Escherichia coli.

Materials and Methods: The antibacterial effect of methanolic extract of Anacyclus pyrethrum and Pistacia lentiscus L. were examined in disk diffusion and skipped wells methods by measuring the diameter of inhibition zones around wells containing different concentrations of extracts from (10-1000 mg/ml) using standard broth macrodilution, method the MIC and MBC were defined.

Results: The methanolic extract of Anacyclus pyrethrum from 300 to 1000 mg/ml and the methanolic extract of Pistacia lentiscus L. from 30 to 1000 mg/ml showed antibacterial activity on Escherichia coli. The MIC of Anacyclus pyrethrum and Pistacia lentiscus L. methanolic based extract were 800 and 1000 mg/ml, respectively. The MBC was achieved at 800 mg/ ml for methanolic extract of Anacyclus pyrethrum and Pistacia lentiscus L.

Conclusion: The methanolic extract of Anacyclus pyrethrum and Pistacia lentiscus L. have antibacterial effect on Escherichia coli bacteria. This activity is dose-dependent.

Keywords: Antibacterial activity, Anacyclus pyrethrum, Pistacia lentiscus L., Escherichia coli


Keywords


Antibacterial activity;Anacyclus Pyrethrum; Pistacia lentiscus L; Escherichia coli

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References


Singleton P (1999). Bacteria in Biology, Biotechnology and Medicine. Wiley, Hoboken. pp. 444-454.

Abduzaimovic A , Aljicevic M , Rebic V , Vranic SM, Abduzaimovic K , Sestic S . Antibiotic resistance in urinary isolates of Escherichia coli. Mater Sociomed 2016;28 :416-419.

Brooks GF. Carroll KC , Butel JS, Morse SA, Mietzner TA (2010). In: Jawetz, Melnick, & Adelberg's Medical Microbiology. New York: McGraw-Hill, pp. 217-218,354.

Zonyane S, Van Vuuren SF, Makunga NP. Antimi- crobial interactions of Khoi-San poly-herbal remedies with emphasis on the combination; Agathosma cren- ulata, Dodonaea viscosa and Eucalyptus globulus. J Soroush, Tehran. pp. 338-358. (In Persian)

Usmani A, Khushtar M, Muhammad Arif, Siddiqui M.A, Prakash Sing S, Mujahid M. Pharmacognostic and phytopharmacology study of Anacyclus pyre- thrum: An insight. J App Pharm Sci 2016; 6:144-150.

Sujith K, Ronald Darwin C, Suba V. Antioxidant activ- ity of ethanolic root extract of Anacyclus pyrethrum. Int Res J Pharm 2011;2:222-226.

Pandey V, Agrawal V, Raghavendra K, Dash AP.Strong larvicidal activity of three species of Spilanthes (Akarkara) against malaria (Anopheles stephensi Lis- ton, Anopheles culicifacies, species C) and filaria vec- tor (Culex quinquefasciatus Say). Parasitol Res 2007;102: 171-174.

Selles C, Dib Mel A, Djabou N, Beddou F, Muselli A, Tabti B, et al. Antimicrobial activity and evolution of the composition of essential oil from Algerian Anacy- clus pyrethrum L. through the vegetative cycle. Nat Prod Res 2013; 27:2231-2234.

Zargari A. Medicinal plants. 4th ed. Tehran: Tehran University Publications; 1985:569-71. (In Persian)

Paraschos S, Mitakou S, Skaltsounis AL Chios gum mastic: A review of its biological activities. Curr Med Chem 2012; 19(14):2292-302.

Dabos KJ, Sfika E, Vlatta LJ, Giannikopoulos G.The effect of mastic gum on Helicobacter pylori:

a randomized pilot study. Phytomedicine 2010; 17(3-4):296-9.

Mahon CR, Lehman C L, Manuselis G .The text book of Diagnostic Microbiology .5thed, Elsevier ; 2015:274-312.

Clinical and Laboratory Standards Institute (2012). (CLSI). M100-S22.Performance standards for antimi- crobial susceptibility testing. CLSI, 22rd informational supplement, Wayne, PA, USA.

Jalayer Naderi N, Niakan M, Mohamadi Motlagh M.Determination of antibacterial activity of Pistacia len-tiscus methanolic extract on Staphylococcus aureus, Streptococcus mutans, Streptococcus sanguis, Pseu- domonas aeruginosa. JIUMS 2015; 22 : 67-74. (In Per- sian)

Takahashi K, Fukazawa M, Motohira H, Ochiai K, Ni- shikawa H, Miyata T. A pilot study on antiplaque ef- fects of mastic chewing gum in the oral cavity. J Peri- odontol 2003;74:501-505.

Aksoy A, Duran N, Koksal F. In vitro and in vivo anti- microbial effects of mastic chewing gum against Strep- tococcus mutans and mutans streptococci. Arch Oral Biol 2006; 51:476-481.

Koutsoudaki C , Krsek M, Rodger A. Chemical com- position and antibacterial activity of the essential oil and the gum of Pistacial entiscus Var. chia. J Agric Food Chem 2005; 53(20):7681-5.

Sakagami H, Kishino K, Kobayashi M, Hashimoto K, Iida S, Shimetani A, et al. Selective antibacterial and apoptosis-modulating activities of mastic. In Vivo 2009; 23:215-223.

Mharti FZ , Lyoussi B, Abdellaoui A. Antibacterial ac- tivity of the essential oils of Pistacia lentiscus used in Moroccan folkloric medicine. Nat Prod Commun 2011;6:1505-1506


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