Probiotic Lactobacillus plantarum IS 10506 supplementation increase SCFA of women with functional constipation

  • Pratiwi Dyah Kusumo Biomedic Doctoral Programme, School of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Biomedic, School of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia
  • Hasan Maulahela Department of Gastroenterology, School of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Amanda Pitarini Utari Department of Gastroenterology, School of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Ingrid S. Surono Department of Food Technology, Faculty of Engineering, Bina Nusantara University, Jakarta, Indonesia
  • Amin Soebandrio Eijkman Biology Molecular Institute, Jakarta, Indonesia; Department of Microbiology, School of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Murdani Abdullah Department of Gastroenterology, School of Medicine, Universitas Indonesia, Jakarta, Indonesia
Keywords: Short chain fatty acid; Lactobacillus plantarum; Functional constipation; Probiotic

Abstract

Background and Objectives: Gut microbiota influences our health via multiple mechanisms. Microbiota produced Short Chain Fatty Acid (SCFA) as an energy to maintain gut ecosystem and physiology. Dysbiosis is correlated with SCFA imbalance which in turn resulted in physiological abnormalities in the intestine, such as functional constipation.
Materials and Methods: Randomized Double-Blind Controlled Trial (RCT) was conducted on women with functional constipation (n=37) in the community of Jakarta and profile of SCFA was assessed by using GC-MS from the stool after 21 days supplementation of fermented milk (placebo and probiotic).
Results: Probiotic supplementation significantly influenced acetate titer (p=0,032) marginally significant for propionate and butyrate (p=0.063 and p=0.068, respectively) and the respondent with increasing SCFA’s metabolite are higher in probiotic group compared to the respondents in placebo group. Acetate is the highest SCFA titer found in faeces samples of women with functional constipation.
Conclusion: Probiotic Lactobacillus plantarum IS 10506 supplementation influenced all the SCFA parameter (acetate, propionate and butyrate).

References

1. Indonesian Society of Gastroenterology (ISG). National consensus on the management of constipation in indonesia 2010. Acta Med Indones 2011; 43: 267-274.
2. Zhao Y, Yu Y-B. Intestinal microbiota and chronic constipation. Springerplus 2016; 5:1130.
3. Drossman DA, Hasler WL. Rome IV-Functional GI disorders: Disorders of gut brain interaction. Gastroenterology 2016; 150:1257-1261.
4. Ivanov II, Littman DR. Segmented filamentous bacteria take the stage. Mucosal Immunol 2010; 3:209-212.
5. Ewald N. (2016). Analysis of short chain fatty acids in faecal samples. Swedish University of Agricultural Sciences, Department of Food Science. Uppsala. Swedish.
6. Iebba V, Totino V, Gagliardi A, Santangelo F, Cacciotti F, Trancassini M, et al. Eubiosis and dysbiosis: the two sides of the microbiota. New Microbiol 2016; 39:1-12.
7. Kim CH, Park J, Kim M. Gut microbiota-derived short-chain fatty acids, T cells, and inflammation. Immune Netw 2014; 14: 277-288.
8. Parthasarathy G, Chen J, Chen X, Chia N, O’Connor HM, Wolf PG, et al. Relationship between microbiota of the colonic mucosa vs feces and symptoms, colonic transit, and methane production in female patients with chronic constipation. Gastroenterology 2016: 150:367-379.e1.
9. Collado MC, Surono I, Meriluoto J, Salminen S. Indigenous dadih lactic acid bacteria: Cell-surface properties and interactions with patogens. J Food Sci 2007; 72:M89-93.
10. Seong-Eun K. Colonic slow transit can cause changes in the gut environment observed in the Elderly. J Neurogastroenterol Motil 2017; 23:3-4.
11. Bellini M, Usai-Satta P, Bove A, Bocchini R, Galeazzi F, Battaglia E, et al. Chronic constipation diagnosis and treatment evaluation: the “CHRO.CO.DI.T.E.” study.BMC Gastroenterol 2017;17:11.
12. Koliada A, Syzenko G, Moseiko V, Budovska L, Puchkov K, Perederiy V, et al. Association between body mass index and Firmicutes / Bacteroidetes ratio in an adult. BMC Microbiol 2017;17:120.
13. Louis P, Flint HJ. Formation of propionate and butyrate by the human colonic microbiota. Environ Microbiol 2017; 19:29-41.
14. Meerveld BG, Johnson AC, Grundy D. Gastrointestinal Physiology and Function. 2017; Springer International Publishing AG.
15. Tobe T, Nakanishi N, Sugimoto N. Activation of motility by sensing short-chain fatty acids via two steps in a flagellar gene regulatory cascade in enterohemorrhagic Escherichia coli. Infect Immun 2011; 79:1016-1024.
16. Koh A, Vadder DF, Kovatcheva-Datchary P, Ba¨ckhed F. From dietary fiber to host physiology: short-chain fatty acids as key bacterial metabolites. Cell 2016;165:1332-1345.
17. Teixeira TF, Grześkowiak Ł, Franceschini SC, Bressan J, Ferreira CL, Peluzio MC. Higher level of faecal SCFA in women correlates with metabolic syndrome risk factors. Br J Nutr 2013: 109:914-919.
18. Farup PG, Rudi K, Hestad K. Faecal short-chain fatty acids-a diagnostic bioprofile for irritable bowel syndrome?. BMC Gastroenterol 2016;16:51.
19. Besten G , Eunen K, Groen AK, Venema K, Reijngoud DJ, Bakker BM. The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. J Lipid Res 2013;54:2325-2340.
20. Venegas DP, De la Fuente MK, Landskron G, González MJ, Quera R, Dijkstra G, et al. Short chain fatty acids (SCFAs)-mediated gut epithelial and immune regulation and its relevance for inflammatory bowel diseases. Front Immunol 2019: 10: 277.
21. Haenen D, Zhang J, Souza da Silva C, Bosch G, van der Meer IM, van Arkel J, et al. A diet high in resistant starch modulates microbiota composition, SCFA concentrations, and gene expression in pig intestine. J Nutr 2013: 143:274-283.
22. Huda-Faujan N, Abdulamir AS, Fatimah AB, Anas OM, Shuhaimi M, Yazid AM, et al. The impact of the level of the intestinal short chain fatty acids in inflammatory bowel disease patients versus healthy subjects. Open Biochem J 2010; 4:53-58.
23. Nourrisson C, Scanzi J, Pereira B, NkoudMongo C, Wawrzyniak I, Cian A, et al. Blastocystis is associated with decrease of fecal microbiota protective bacteria: comparative analysis between patients with irritable bowel syndrome and control subjects. PLoS One 2014; 9(11):e111868.
24. Chassard C, Dapoigny M, Scott KP, Crouzet L, Del'homme C, Marquet P, et al. Functional disbiosis within the gut microbiota of patients with constipated-irritable bowel syndrome. Aliment Pharmacol Ther 2012; 35:828-838.
25. Quigley EMM, Spiller RC. Constipation and the microbiome: Lumen Versus Mucosa! Gastroenterology 2016; 150:300-303.
26. Ríos-Covián D, Ruas-Madiedo P, Margolle A, Gueimonde M, De los Reyes-Gavilán CG, Salazar N. Intestinal short chain fatty acids and their link with diet and human health. Front Microbiol 2016; 7: 185.
27. Esquivel-Elizondo S, Ilhan ZE, Garcia-Peña EI, Krajmalnik-Brown, R. Insights into butyrate production in a controlled fermentation system via gene predictions. mSystems 2017; 2(4): e00051-17.
28. Hamer HM, Jonkers D, Venema K, Vanhoutvin S, Troost FJ, Brummer RJ. Review article: the role of butyrate on colonic function. Aliment Pharmacol Ther 2008; 27: 104-119.
29. Wang L, Zhang J, Guo Z, Kwok L, Ma C, Zhang W, et al. Effect of oral consumption of probiotic Lactobacillus planatarum P-8 on fecal microbiota, SIgA, SCFAs, and TBAs of adults of different ages. Nutrition 2014; 30:776-783.e1.
30. Sun Q, Jia Q, Song L, Duan L. Alterations in fecal short-chain fatty acids in patients with irritable bowel syndrome: A systematic review and meta-analysis. Medicine (Baltimore) 2019; 98(7):e14513.
31. Gargari G, Taverniti V, Gardana C, Cremon C, Canducci F, Pagano I, et al. Fecal Clostridiales distribution and short-chain fatty acids reflect bowel habits in irritable bowel syndrome. Environ Microbiol 2018: 20:3201-3213.
32. Ibarra A, Latreille-Barbierb M, Donazzolob Y, Pelletierb X, Ouwehand AC. Effects of 28-day Bifidobacterium animalis subsp. lactis HN019 supplementation on colonic transit time and gastrointestinal symptoms in adults with functional constipation: A double-blind, randomized, placebo-controlled, and dose-ranging trial. Gut Microbes 2018; 9: 236-251.
33. Husebye E, Hellstrom PM, Sundler F, Chen J, Midtvedt T. Influence of microbial species on small intestinal myoelectric activity and transit in germ-free rats. Am J Physiol Gastrointest Liver Physiol 2001;280: G368-380.
34. Bercik P, Collins SM, Verdu EF. Microbes and the gut-brain axis. Neurogastroenterol Motil 2012: 24:405-413.
35. Dimidi E, Christodoulides S, Scott M, Whelan K. Mechanisms of action of probiotics and the gastrointestinal microbiota on gut motility and constipation. Adv Nutr 2017: 8:484-494.
36. Jalanka J, Major G, Murray K, Singh G, Nowak A, Kurtz C, et al. The effect of psyllium husk on intestinal microbiota in constipated patients and healthy controls. Int J Mol Sci 2019: 20:E433.
Published
2019-11-12
How to Cite
1.
Kusumo P, Maulahela H, Utari A, Surono I, Soebandrio A, Abdullah M. Probiotic Lactobacillus plantarum IS 10506 supplementation increase SCFA of women with functional constipation. Iran J Microbiol. 11(5):389-396.
Section
Original Article(s)