The combined effects of ethanolic extract of Artemisia aucheri and Artemisia oliveriana on biofilm genes expression of methicillin resistant Staphylococcus aureus

  • Ghazaleh Shojaei Baghini Department of Microbiology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
  • Abbas Akhavan Sepahi Department of Microbiology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
  • Robab Rafiei Tabatabaei Department of Microbiology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
  • Kambiz Tahvildari Department of Chemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran
Keywords: Artemisia aucheri, Artemisia oliveriana, Biofilm, Gene expression, Staphylococcus aureus

Abstract

Background and Objectives: One of the most important antibiotic-resistant bacteria is methicillin-resistant Staphylococcus aureus (MRSA) biofilm that has caused significant problems in treating the patients. Therefore, the aim of this study was to evaluate the levels of expression of genes involved in biofilm formation in MRSA (ATCC 33591) while being treated by a combination of Artemisia aucheri and Artemisia oliveriana.Materials and Methods: The minimum inhibitory concentration (MIC) of ethanolic extract of A. aucheri and A. oliveriana and also the minimum inhibitory concentration of combination of both extracts were 512, 1024 and 256 µg/ml, respectively; then at concentrations lower than the MIC, expression levels of the desired genes were determined by Real Time PCR. Results: Based on results, using a combination of two ethanolic extracts had a significant effect on expression of genes involved in biofilm formation in MRSA. The expression level of icaA at 4, 8, 16 h after being treated by herbal extracts of A. aucheri and A. oliveriana was 0.293, 0.121, 0.044, respectively. The expression level of icaD was 0.285, 0.097, 0.088, respectively, while that of ebps was 0.087, 0.042, 0.009 at 4, 8 and 16 h, respectively.Conclusion: This study provided evidence that ethanol extract of A. oliveriava and A. aucheri can inhibit the biofilm formation of S. aureus. As a traditional Iranian medicine, A. oliveriava and A. aucheri extracts have a potential antibiofilm formation against MRSA strains.

References

References

Lora-Tamayo, J., Murillo, O., Iribarren, JA., Soriano, A., Sánchez-Somolinos, M., Baraia-Etxaburu, JM.,(2013), A large multicenter study of methicillin-susceptible and methicillin-resistant Staphylococcus aureus prosthetic joint infections managed with implant retention. Clin Infect Dis; 56(2),182–94.

Salgado, CD., Dash, S., Cantey, JR., Marculescu, CE., (2007),Higher risk of failure of methicillin-resistant Staphylococcus aureus prosthetic joint infections. Clin Orthop Relat Res; 461, 48–53.

Costerton, JW., Stewart, PS., Greenberg, EP., (1999),Bacterial biofilms: a common cause of persistent infections. Science . 284(5418), 1318–1322.

Swan, A., Amer, H., Dieppe, P., (2002),The value of synovial fluid assays in the diagnosis of joint disease: a literature survey. Ann Rheum Dis; 61(6), 493–498.

Simon, GL., Miller, HG., Borenstein, DG., (1983),Synovial fluid inhibits killing of Staphylococcus aureus by neutrophils. Infect Immun; 40(30),1004–1010.

Dastgheyb, S., Parvizi, J., Shapiro, IM., Hickok, NJ., Otto1, M,.(2015), Effect of Biofilms on Recalcitrance of Staphylococcal Joint Infection to Antibiotic Treatment. J Infectious Dis; 211(4), 641–650.

Smith-Palmer, A., Stewart, J., Fyfe, L., (2001),The potential application of plant essential oils as natural food preservatives in soft cheese. Food Microbiol; 18(4),463-470.

Amin ,G. (1991),Traditional medicinal plants of Iran. Ministry of health, Treatment and medical education, Tehran Press; p. 69. [Text in Persian]

Das, S., Pal, S., Mujib, A.,(1999) Biotechnology of medicinal plants recent advances and potential. Hyderabad. UK 992 Press; p. 126-39.

Rechinger, KH., Hedge, IC.,(1986), editors. Compositae. Austria: AkademischeDruck and Verlagsanatalt, Grazp. 214.

Inouye, S., Takizawa, T., Yamaguchi, H.,(2001), Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. J Antimicrob Chemother,47:565.

Nuryastuti ,T., van der Mei, HC., Busscher, HJ., Iravati, S., Aman, AT., Krom, BP.,(2009), Effect of cinnamon oil on icaA expression and biofilm formation by Staphylococcus epidermidis. Appl Environ Microbiol;75(21),6850-6855.

Kim, YG., Lee JH, Kim, S Il., Baek, KH., Lee, J., (2015),Cinnamon bark oil and its components inhibit biofilm formation and toxin production. Int J Food Microbiol;195,30-39.

Zhang, Y., Liu, X., Wang, Y., Jiang, P., Quek, S., (2015),Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control;59,282-289.

Otto, M.,(2008),Staphylococcus aureus biofilms.curr Top Microbial Immunol;322,:20-28.

Moreno, S.,Galva’n, E.M.,Va’zquez N,Fiorilli, G.,Guido Ca’ceres, P.A.,(2015).Antibacterial efficacy of Rosmarinus officinalis phytochemical against nosocomial multidrug resistant bacteria grown in planktonic culture and biofilm. The Battle Against Microbial.

Yang chio, Na,,Yang Kang,Kang-Ju Kim.,(2015),Artemisia princeps Inhibits Biofilm Formation and Virulance-Factor Expression of Antibiotics-Resistant Bacteria.Biomed Research International.

Esmaeili, D.,Bahador, A,Saghii, H.,Ataee, R,.(2015), The Study of Inhibition Effects Satureja khuzestaniae Essence against Expression bap Acinetobacter baumannii with Real Time PCR Technique.Iran J med Microbial;1394(1),42-49.

Published
2019-02-10
How to Cite
1.
Shojaei Baghini G, Akhavan Sepahi A, Rafiei Tabatabaei R, Tahvildari K. The combined effects of ethanolic extract of Artemisia aucheri and Artemisia oliveriana on biofilm genes expression of methicillin resistant Staphylococcus aureus. Iran J Microbiol. 10(6):417-23.
Section
Original Article(s)