Tracking leading anti-Candida compounds in plant samples; Plumbago europaea

  • Marzieh Sobhani Department of Phytochemistry and Essential Oils Technology, Factually of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
  • Mahdi Abbas-Mohammadi Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
  • Samad Nejad Ebrahimi Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
  • Atousa Aliahmadi Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran http://orcid.org/0000-0002-1723-3914
Keywords: Bio-autography, Plumbagin, Candida albicans, Gardnerella vaginalis

Abstract

Background and Objectives: Due to the importance of finding new and more effective antifungal and antibacterial compounds against invasive vaginitis strains, this study was conducted for fast screening of plant samples.Materials and Methods: Thirty Iranian plant samples were successively extracted by n-hexane, ethyl acetate and methanol to obtain a total of 90 extracts. Each extract was prepared in six concentrations and evaluated for antifungal activity via a micro-broth dilution method. Further phytochemical study of the aerial parts of Plumbago europaea, as the most promising source of anti-Candida compounds (with minimum inhibitory concentration of about 7µg/ml), was carried out and antifungal activity in the ethyl acetate extract was tracked using a combination of HPLC time-based fractionation and Thin Layer Chromatography-Bioautography via a bioassay-guided fractionation procedure. The compounds in the active region of the chromatogram were purified by a combination of column chromatography and preparative TLC, and then structure elucidation was achieved by 1D and 2D NMR, mass spectrometry and UV spectra.Results: Seven compounds were isolated and identified: (1) plumbagin, (2) isoplumbagin, (3) 5, 8-dihydroxy-2-methyl-[1, 4] naphthoquinone, (4) droserone, (5) 7-methyljuglone, (6) Isozeylanone, and (7) methylene-3, 3’-diplumbagin. Antimicrobial activity of the purified compounds were also evaluated against C. albicans (MIC values ranging from 2 to 2500 µM) and Gardnerella vaginalis (MIC values ranging from 20 to 2500 µM).Conclusion: These naphthoquinone compounds could be surveyed for finding new and more effective anti-vaginitis agents via drug design approaches.

Author Biography

Atousa Aliahmadi, Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
Assistant professor

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Published
2018-07-15
How to Cite
1.
Sobhani M, Abbas-Mohammadi M, Nejad Ebrahimi S, Aliahmadi A. Tracking leading anti-Candida compounds in plant samples; Plumbago europaea. IJM. 10(3):187-93.
Section
Original Article(s)