Iranian Journal of Microbiology 2018. 10(1):59-64.

Serological and molecular characterization of hepatitis B virus in asymptomatic blood donors in Iran
Mona Seyed Attaran, Seyed Masoud Hosseini, Javad Fakhari, Zohreh Sharifi

Abstract


Background and Objectives: Hepatitis B virus (HBV) is an enveloped DNA virus belongs to Hepadnaviridea family. HBV infection is a serious global health problem, with 2 billion people infected worldwide, and 350 million suffering from chronic HBV infection. The aim of this study was to determine the serological pattern and molecular characterization of HBV diversity in asymptomatic blood donors in Iran alongside with the mutation status of HBV in relation to blood safety.


Materials and Methods: One hundred and sixty six samples from asymptomatic blood donors who were positive for hepatitis B surface antigen during 2012 to 2014 were selected. The serological and molecular markers were analyzed by screening HBsAb, HBcAb, HBeAg and HBeAb and HBV-DNA. For detection of HBV genotypes and possible mutations, HBV polymerase and pre core/core regions were sequenced.


Results: In term of serologic markers of HBV, 100% of asymptomatic blood donors were HBsAg positive and 97.6%, 92.2%, 5.4% and 2.4% of them were HBcAb, HBeAb, HBeAg and HBsAb positive respectively in asymptomatic blood donors. The maximum of samples viral load was 4.41 × 107 IU/ml for HBeAg positive blood donors. While the minimum and maximum of viral load in HBeAb positive samples was 1.21 × 102 IU/ml and 1 × 106 IU/ml respectively and the mean of viral load in HBeAb positive samples was 5.882 × 103 IU/ml. About 9.7% of HBeAb positive samples had a pre core mutation that is related to stopping the synthesis of HBeAg and only genotype D was prevalent in asymptomatic blood donors.


Conclusion: This study showed that from 166 samples most of them were in a chronic phase of HBV infection and just 5.4% of asymptomatic blood donors were in the acute phase or acute chronic phase of HBV infection. The major risk factor for HBV infection was a familial history of HBV.


Keywords


Hepatitis B virus, Serological markers, Viral load, Iran

Full Text:

PDF

References


Dienstag JL, Acute Viral Hepatitis. In: Longo DL, Fauci AS, editors. Harrison’s Gastroenterology and Hepatology. New York: McGraw-Hill Company, Inc, New York, 2010: 349-77.

Kawsar HI, Shahnewaz J, Gopalakrishna KV, Spiro TP, Daw HA. Hepatitis B reactivation in cancer patients: Role of prechemotherapy screening and antiviral prophylaxis. Clin Adv Hematol Oncol 2012; 10: 370-378.

Hou J, Liu Z, Gu F. Epidemiology and preven-tion of hepatitis B virus infection. Int J Med Sci 2005; 2:50-57.

Alavian SM. Hepatitis B virus infection in Iran; changing the epidemiology. Iran J Clin Infect Dis 2010; 5:51-61.

Mahmoodian-Shooshtari M, Sharifi Z, Mousavi SE, Ahmadi-Teymourloyee SA. Decline of hepatitis B virus in Iranian blood donors in the last decade (2001-2010). Iran J Virol 2012; 6: 8-12.

Karimi GH, Zadsar M, Vafaei N, Sharifi Z, Tafti MF. Prevalence of antibody to hepatitis B core antigen and hepatitis B virus DNA in HBsAg negative healthy blood donors. Virol J 2016; 13: 36.

Alizadeh Z, Milani S , Sharifi Z. Occult hepatitis B virus infection among Iranian blood donors: a preliminary study. Arch Iran Med 2014; 17: 106-107.

Attaran MS, Sharifi Z, Hosseini SM, Samei S, Ataee Z. Prevalence of hepatitis B and hepatitis D coinfection in asymptomatic blood donors in Iran. APMIS 2014; 122: 243-247.

Alavian SM, Fallahian F, Bagheri Lankarani K. The changing epidemiology of viral Hepatitis B in Iran. J Gastrointestin Liver Dis 2007; 16: 403-406.

Brian JM. The natural history of chronic hepatitis B virus infection. Hepatology 2009; 49(5 Suppl):S45-55.

Jesmin Rabbi F, Rezwan K, Shirin T. HBeAg/anti-HBe, alanine aminotransferase and HBV DNA levels in HBsAg positive chronic carriers. Bangladesh Med Res Counc Bull 2008; 34: 39-43.

Milani S, Sharifi Z, Hosseini M, Mahmoodian Shooshtari M. Determination of HBV genotypes among Hbs Ag positive blood donors in Tehran, Iran using PCR-RFLP. Iran J Publ Health 2009; 38: 41-47.

Ghabeshi S, Sharifi Z, Hosseini SM, Mahmoodian Shooshtari M. Correlation between viral load of HBV in chronic hepatitis B patients and precore and basal core promoter mutations. Hepat Mon 2013; 13(2): e7415.

Rosenberg GK, Lattimore S, Brailsford SR, Hewitt PE, Tettmar KI, Kitchen AD, et al. The diversity of chronic hepatitis B virus infections within blood donors in England and North Wales 2005 through 2010. Transfusion 2013; 53(10 Pt 2):2467-2476.

Servant-Delmas A, Mercier M, El Ghouzzi MH, Girault A, Bouchardeau F, pillonel J, et al. National survey of hepatitis B virus (HBV) polymorphism in asymptomatic HBV blood donorsfrom 1999 to 2007 in France. Transfusion 2010; 50 :2607-2618.

Amini kafi-abad S, Talebian A. Hepatitis B virus infection in an anti-HBc negative patient: a case report. Med J Islam Repub Iran 2006; 20: 98-100.

Wang L, Liu H, Ning X, Gaohepr F. Sequence analysis of the S gene region in HBV DNA from patients positive for both HBsAg and HBsAb tests. Hepatol Res 2010; 40: 1212-1218.

Seed CR, Jones NT, P ickworth AM, Graham WR. Two cases of asymptomatic HBV "vaccine breakthrough" infection detected in blood donorsscreened for HBV DNA. Med J Aust 2012; 196:651-652.

Sharifi Z, Yari F, Gharebaghiyan A. Sequence analysis of the polymerase gene in hepatitis B virus infected blood donors in Iran. Arch Iran Med 2012; 15:88-90.

Ruiz-Tachiquín ME, Valdez-Salazar HA, Juarez-Barreto V, Dehesa-violante M, Torres J, Munoz-Hernandes O, et al. Molecular analysis of hepatitis B virus "a" determinant in asymptomatic and symptomatic Mexican carriers. Virol J 2007; 4:6.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.