Iranian Journal of Microbiology 2018. 10(1):65-71.

Seroprevalence and genotyping of avian infectious bronchitis virus detected from Iranian unvaccinated backyard chickens
Shima Shokri, Vahid Karimi, Arash Ghalyanchi Langeroudi, Mehdi Vasfi Marandi, Masoud Hashemzadeh, Taha Zabihipetroudi, Hamideh Najafi, Farshad Tehrani


Background and Objectives: Different epidemiological studies have found that backyard chickens are a reservoir for poultry diseases. Most backyard chicken flocks have a poor level of biosecurity, which increases the risk of spread of diseases. In recent years, the number of backyard chickens has been on the rise in Iran. However, the health status of backyard flocks is still poorly documented. Thus, this study aimed at examining the seroprevalence of antibodies against infectious bronchitis virus (IBV) and molecular surveillance and genotyping of IBV among backyard chickens (without vaccination history) in Mazandaran province, North of Iran, 2014.

Materials and Methods: A total of 460 blood samples of unvaccinated backyard chickens in the mentioned area were tested for antibodies against IBV using commercial ELISA. Also, cecal tonsils were collected from 75 chickens in the same area. Real time RT-PCR (for detection) and RT-PCR and sequencing spike gene were performed.

Results: The seropositivity rate was 54.5%. In addition, we detected 793/B, Variant 2, and QX in the backyard flocks and performed phylogenetic studies on them. The phylogenetic study revealed that the detected genotypes had high homology with IBV strains that were infected broilers, pullets, and layers in Iran.

Conclusion: There is a need for continuous monitoring of IBV among avian species to complete the epidemiological map and work on the pathogenesis of Iranian IBV strains in Iranian backyard chickens.


Avian infectious bronchitis, Backyard chicken, Phylogenetic, Spike, Iran

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Cavanagh D. Coronavirus avian infectious bronchitis virus. Vet Res 2007;38:281-297.

Umar S, Shah M, Munir M, Ahsan U, Kaboudi K. Infectious bronchitis virus: evolution and vaccination. Worlds Poult Sci J 2016;72:49-60.

Promkuntod N, van Eijndhoven RE, de Vrieze G, Gröne A, Verheije MH. Mapping of the receptor-binding domain and amino acids critical for attachment in the spike protein of avian coronavirus infectious bronchitis virus. Virology 2014;448:26-32.

Kamble NM, Pillai AS, Gaikwad SS, Shukla SK, Khulape SA, Dey S, et al. Evolutionary and bioinformatics analysis of the spike glycoprotein gene of H120 vaccine strain protectotype of infectious bronchitis virus from India. Biotechnol Appl Biochem 2016;63:106-112.

Cook JK, Jackwood M, Jones RC. The long view: 40 years of infectious bronchitis research. Avian Pathol 2012;41:239-250.

Jackwood MW. Review of infectious bronchitis virus around the world. Avian Dis 2012;56:634-641.

Cavanagh D. Coronaviruses in poultry and other birds. Avian Pathol 2005;34:439-448.

Sjaak de Wit JJ, Cook JK, van der Heijden HM. Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol 2011;40:223-235.

Seyfi Abad Shapouri M, Mayahi M, Assasi K, Charkhkar S. A survey of the prevalence of infectious bronchitis virus type 4/91 in Iran. Acta Vet Hung 2004;52:163-166.

Mahzounieh M, Karimi I, Bouzari M, Salehi T, Iravani S. A serological survey for detection of avian infectious bronchitis virus antibodies in domestic village chickens in Esfahan, central Iran. Iran J Vet Res 2006;7:89-91.

Al-Shekaili T, Baylis M, Ganapathy K. Molecular detection of infectious bronchitis and avian metapneumoviruses in Oman backyard poultry. Res Vet Sci 2015;99:46-52.

Saadat Y, Ghafouri SA, Tehrani F, Langeroudi AG. An active serological survey of antibodies to newcastle disease and avian influenza (H9N2) viruses in the unvaccinated backyard poultry in Bushehr province, Iran, 2012-2013. Asian Pac J Trop Biomed 2014;4(Suppl 1):S213-216.

Callison SA, Hilt DA, Boynton TO, Sample BF, Robison R, Swayne DE, et al. Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens. J Virol Methods 2006;138(1-2):60-65.

Najafi H, Langeroudi AG, Hashemzadeh M, Karimi V, Madadgar O, Ghafouri SA, et al. Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran, 2014-2015. Arch Virol 2016;161:53-62.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-2739.

Langeroudi AG, Hosseini H, Karimi V, Hashemzadeh M, Estabragh AS, Madadgar O. Phylogenetic study base on matrix gene of Iranian Newcastle disease virus isolates, 2011-2012. Comp Clin Path 2014:23;77-81.

Hadipour M, Azad F, Vosoughi A, Fakhrabadipour M, Olyaie A, Kazerun I. Measurement of antibodies to infectious bronchitis virus in indigenous chicken flocks around maharlou lake in Iran. Int J Anim Vet Adv 2011;3(3):182-185.

Akbari Azad G, Vasfi Marandi M, Keyvani Aminae H. Molecular analysis of three Iranian isolates belonged to 793/B serotype of infectious bronchitis viruses. J Vet Res 2007;62:69-80.

Bozorgmehri-Fard M, Charkhkar S, Hosseini H. Detection of the Chinese genotype of infectious Bronchitis Virus (QX-type) in Iran. Iran J Virol 2013;7(1&2):57-61.

Gelb Jr J, Weisman Y, Ladman BS, Meir R. S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel (1996 to 2000). Avian Pathol 2005;34:194-203.

Abdel-Moneim AS, Afifi MA, El-Kady MF. Emergence of a novel genotype of avian infectious bronchitis virus in Egypt. Arch Virol 2012;157:2453-2457.


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