Iranian Journal of Microbiology 2017. 9(3):169-173.

Prevalence of Clostridium difficile and its toxigenic genotype in beef samples in West of Iran
Malihe Kheradmand, Somayeh Jalilian, Amirhooshang Alvandi, Ramin Abiri

Abstract


Background and Objectives: Clostridium difficile is the leading cause of nosocomial diarrhea and pseudomembranous colitis. The prevalence of C. difficile infection differs in various geographical areas. The aim of this study was to determine the prevalence of C. difficile isolates and the prevalence of cdd3, tcdA and tcdB genes in beef samples in Kermanshah Province.
Materials and Methods: One hundred ground beef samples were randomly collected from the butchers of Kermanshah province during March 2014 to March 2015. Following alcohol shock, minced meat samples were incubated in a specific culture medium for 5 to 7 days. The suspicious colonies were analyzed by biochemical tests and frequency of C. difficile and cdd3, tcdA and tcdB genes was assessed by PCR using specific primers.
Results: In total, 30% samples were positive for C. difficile and all the isolates harbored Cdd3 gene. Combined dual-gene frequency of A+B+, A-B+ and A+B- strains in the positive were 0%, 3.3%, and 26.6% respectively, while 21 samples (70%) were non-toxigenic (A-B-).
Conclusion: In this study, the presence of C. difficile in beef as a source of contamination was confirmed. It was also shown that the incidence of C. difficile in ground meat was relatively higher than many other studies.


Keywords


Clostridium difficile, Ground beef, PCR

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References


Paredes-Sabja D, Shen A, Sorg JA. Clostridium difficile spore biology: sporulation, germination, and spore structural proteins. Trends Microbiol 2014; 22:406-416.

Pelleschi ME. Clostridium difficile-associated disease: diagnosis, prevention, treatment, and nursing care. Crit Care Nurse 2008; 28: 27-35.

Hall I, O'Toole E. Intestinal microflora in newborn infants with a description of a new pathogenic anaerobe, Bacillus difficilis. Am J Dis Child 1935; 49: 390-402.

Kelly CP, Pothoulakis C, LaMont JT. Clostridium difficile colitis. N Engl J Med 1994; 330: 257-262.

Rubin MS, Bodenstein LE, Kent KC. Severe Clostridium difficile colitis. Dis Colon Rectum 1995; 38(4): 350-354.

Dial S, Alrasadi K, Manoukian C, Huang A, Menzies D. Risk of Clostridium difficile diarrhea among hospital inpatients prescribed proton pump inhibitors: cohort and case-control studies. Can Med Assoc J 2004;171:33-38.

Kato H, Kato N, Katow S, Maegawa T, Nakamura S, Lyerly DM. Deletions in the repeating sequences of the toxin A gene of toxin A-negative, toxin B-positive Clostridium difficile strains. FEMS Microbiol Lett 1999;175: 197-203.

Keel K, Brazier JS, Post KW, Weese JS, Songer JG. Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. J Clin Microbiol 2007;45:1963-1964.

Rupnik M. Is Clostridium difficile-associated infection a potentially zoo- notic and foodborne disease? Clin Microbiol Infect 2007;13:457-459.

Lyerly DM, Krivan HC, Wilkins TD. Clostridium Difficile: its Diseases and Toxins. Clin Microbiol Rev 1998; 1:1-18.

Kuehne SA, Cartman ST. Both, Toxin A and Toxin B are Important in Clostridium difficile Infection. Gut Microbes 2011; 2(4):252-255.

Riggs MM, Sethi AK, Zabarsky TF, Eckstein EC, Jump RL, Donskey CJ. Asymptomatic Carriers are a Potential Source for Transmission of Epidemic and Non Epidemic Clostridium difficile Strains among long - Term Care Facility Residents. Clin Infect Dis 2007; 45:992-998.

Yoo J, Lightner AL. Clostridium difficile Infections: What Every Clinician Should Know. Perm J 2010; 14: 35-40.

Rogers MA, Greene MT, Saint S, Chenoweth CE, Malani PN, Trivedi I, et al. Higher rates of Clostridium difficile infection among smokers. PLoS One 2012; 7(7): e42091.

Daniel A, Rapose A. The evaluation of Clostridium difficile infection (CDI) in a community hospital. J Infect Public Health 2015; 8:155-160.

Rupnik M, Wilcox MH, Gerding DN. Clostridium difficile infection: new developments in empidemiology and pathogenesis. Nat Rev Microbiol 2009; 7: 526-536.

Guilbault C, Labbe AC, Poirier L, Busque L, Beliveau C, Laverdiere M. Development and evaluation of a PCR method for detection of the Clostridium difficile toxin B gene in stool specimens. J Clin Microbiol 2002; 40: 2288-2290.

Songer JG, Trinh HT, Killgore GE, Thompson AD, McDonald LC, Limbago BM. Clostridium difficile in Retail Meat Products, USA, 2007. Emerg Infect Dis 2009; 15:819-821.

Bouttier S, Barc MC, Felix B, Lambert S, Collignon A, Barbut F. Clostridium difficile in ground meat, France. Emerg Infect Dis 2010; 16:733-735.

Rodriguz-Palacios A, Staemfli H, Duffield T, Weese JS. Clostridium difficile in retail Ground Meat, Canada. Emerg Infect Dis 2007; 13:485-487.

Rahimi E, Jalali M, Weese JS. Prevalence of Clostridium difficile in raw beef, cow, sheep, goat, camel and buffalo meat in Iran. BMC Public Health 2014; 14:119.

Esfandiari Z, Jalali M, Ezzatpanah H, Weese JS, Chamani M. Prevalence and Characterization of Clostridium difficile in Beef and Mutton Meats of Isfahan Region, Iran. Jundishapur J Microbiol 2014; 7(8): e16771.

Alonso R, Martin A, Pelaez T, Marin M, Rodriguez-Creixems M, Bouza E. Toxigenic status of Clostridium difficile in a large Spanish teaching hospital. J Med Microbiol 2005; 54:159-162.

Cobeta-Garcia JC, Domingo-Morera JA, Gracia P. Clostridium difficile-associated diarrhea in a patient with rheumatoid arthritis: comment on the article by Ramos et al. Arthritis Rheum 1998;41:2083-2085.

Kikkawa H, Hitomi S, Watanabe M. Prevalence of toxin A-nonproducing/toxin-B-producing Clostridium difficile in the Tsukuba-Tsuchiura district, Japan. J Infect Chemother 2007;13:35-38.

Persson S, Torpdahl M, Olsen KE. New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA/cdtB) genes applied to a Danish strain collection. Clin Microbiol Infect 2008; 14: 1057-1064.

Rodriguez-Palacios A, Reid-Smith R, Staempfli HR, Weese JS. Colstridium difficile survives minimal temperature recommended for cooking ground meats. Anaerobe 2010; 16: 540-542.

Rodriguez-Palacios A, Lejeune JT. Moist-Heat resistance, spore aging, and superdormancy in Clostridium difficile. Appl Environ Microbiol 2011; 77: 3085-3091.

Lemee L, Dhalluin A, Testelin S, Mattrat M. A, Maillard K, Lemeland JF, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxin A), and tcdB (toxin B) genes for toxigenic culture of Clostridium difficile. J Clin Microbiol 2004; 42: 5710-5714.


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